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2A64

Crystal Structure of Bacterial Ribonuclease P RNA

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU RU200
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date2003-07-01
DetectorRIGAKU RAXIS IV
Spacegroup nameF 2 2 2
Unit cell lengths147.150, 159.420, 410.210
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution37.150 - 3.300
R-factor0.3214
Rwork0.320
R-free0.33990
Structure solution methodMAD
RMSD bond length0.003
RMSD bond angle0.800
Data scaling softwared*TREK
Phasing softwareCNS
Refinement softwareCNS (1.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]500.0003.420
High resolution limit [Å]3.3003.300
Rmerge0.1480.518
Number of reflections35599
<I/σ(I)>7.53.2
Completeness [%]99.499.9
Redundancy5.845.49
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP6.2298(NH4)2SO4, K2SO4, MgCl2, MPD, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1VAPOR DIFFUSION, SITTING DROP6.2298(NH4)2SO4, K2SO4, MgCl2, MPD, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Crystallization Reagents
IDcrystal IDsolution IDreagent nameconcentrationdetails
111(NH4)2SO4
211K2SO4
311MgCl2
411MPD
512(NH4)2SO4
612K2SO4
712MgCl2
812MPD

227561

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