2A4O

Dual modes of modification of Hepatitis A virus 3C protease by a serine derived beta-lactone: selective crytstallization and high resolution structure of the His102 adduct

Summary for 2A4O

• Entry DOI: 10.2210/pdb2a4o/pdb
• Related: 1HAV 1QA7 2CXV
• Descriptor: Probable protein P3C, N-[(BENZYLOXY)CARBONYL]-L-ALANINE, ACETYL GROUP, ... (6 entities in total)
• Functional Keywords: beta barrel, hydrolase
• Biological source: Human hepatitis A virus Hu/Northern Africa/MBB/1978
• Cellular location: Protein VP2: Virion . Protein VP3: Virion . Protein VP1: Virion . Protein VP1-2A: Virion . Protein 2B: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Protein 2C: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Protein 3ABC: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Protein 3AB: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Protein 3A: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Protein 3B: Virion . Protease 3C: Host cytoplasm . RNA-directed RNA polymerase 3D-POL: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side : P13901
• Total number of polymer chains: 1
• Total formula weight: 24668.40

Authors

Yin, J.,Bergmann, E.M.,Cherney, M.M.,Lall, M.S.,Jain, R.P.,Vederas, J.C.,James, M.N.G. (deposition date: 2005-06-29, release date: 2005-12-27, Last modification date: 2021-11-10)

• Primary citation: Yin, J.,Bergmann, E.M.,Cherney, M.M.,Lall, M.S.,Jain, R.P.,Vederas, J.C.,James, M.N.G.; Dual Modes of Modification of Hepatitis A Virus 3C Protease by a Serine-derived beta-Lactone: Selective Crystallization and Formation of a Functional Catalytic Triad in the Active Site; J.MOL.BIOL., 354:854-871, 2005; PubMed: 16288920; DOI: 10.1016/j.jmb.2005.09.074

Experimental method

X-RAY DIFFRACTION (1.55 Å)