4V5G

The crystal structure of the 70S ribosome bound to EF-Tu and tRNA

This is a non-PDB format compatible entry.

Summary for 4V5G

• Entry DOI: 10.2210/pdb4v5g/pdb
• Related: 1DV4 1EG0 1EMI 1FJG 1FKA 1G1X 1GIX 1HA3 1HNW 1HNX 1HNZ 1HR0 1I94 1I95 1I96 1I97 1IBK 1IBL 1IBM 1J5E 1JGO 1JGP 1JGQ 1L1U 1N32 1N33 1N34 1N36 1PN7 1PN8 1PNS 1PNX 1QD7 1QZC 1RSS 1TWT 1VOV 1XMO 1XMQ 1XNQ 1XNR 1YL4 2B64 2B9M 2B9O 2C78 2F4V 2J00 2J02 2JL5 2JL7 2UU9 2UUA 2UUB 2UUC 2UXB 2UXC 2UXD 2V46 2V48 2VQE 2VQF 2WDG 2WDH 2WDK 2WDM 2WH1 2WH3 2WRI 2WRJ 2WRK 2WRL
• Descriptor: 16S RIBOSOMAL RNA, 30S RIBOSOMAL PROTEIN S10, 30S RIBOSOMAL PROTEIN S11, ... (63 entities in total)
• Functional Keywords: ribosomal protein, ribonucleoprotein, nucleotide-binding, protein biosynthesis, translation, zinc-finger, rna-binding, rrna-binding, metal-binding, elongation factor, trna, ef-tu, gtpase, ribosome, decoding
• Biological source: THERMUS THERMOPHILUS; More
• Cellular location: Cytoplasm: Q5SHN6
• Total number of polymer chains: 118
• Total formula weight: 4668785.46

Authors

Schmeing, T.M.,Voorhees, R.M.,Ramakrishnan, V. (deposition date: 2009-09-01, release date: 2014-07-09, Last modification date: 2024-10-23)

Primary citation

Schmeing, T.M.,Voorhees, R.M.,Kelley, A.C.,Gao, Y.G.,Murphy, F.V.,Weir, J.R.,Ramakrishnan, V.
The crystal structure of the ribosome bound to EF-Tu and aminoacyl-tRNA.
Science, 326:688-694, 2009

PubMed Abstract: The ribosome selects a correct transfer RNA (tRNA) for each amino acid added to the polypeptide chain, as directed by messenger RNA. Aminoacyl-tRNA is delivered to the ribosome by elongation factor Tu (EF-Tu), which hydrolyzes guanosine triphosphate (GTP) and releases tRNA in response to codon recognition. The signaling pathway that leads to GTP hydrolysis upon codon recognition is critical to accurate decoding. Here we present the crystal structure of the ribosome complexed with EF-Tu and aminoacyl-tRNA, refined to 3.6 angstrom resolution. The structure reveals details of the tRNA distortion that allows aminoacyl-tRNA to interact simultaneously with the decoding center of the 30S subunit and EF-Tu at the factor binding site. A series of conformational changes in EF-Tu and aminoacyl-tRNA suggests a communication pathway between the decoding center and the guanosine triphosphatase center of EF-Tu.

PubMed: 19833920
DOI: 10.1126/science.1179700

Experimental method

X-RAY DIFFRACTION (3.6 Å)