4V5G
The crystal structure of the 70S ribosome bound to EF-Tu and tRNA
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-14 |
| Detector | ADSC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 289.566, 268.363, 403.884 |
| Unit cell angles | 90.00, 91.01, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.600 |
| R-factor | 0.2808 |
| Rwork | 0.281 |
| R-free | 0.31500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2J00 2J01 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.900 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.220 | 0.770 |
| Number of reflections | 665420 | |
| <I/σ(I)> | 6.15 | 1.73 |
| Completeness [%] | 93.6 | 85.7 |
| Redundancy | 6 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.3 | 100 MM MES PH 6.3, 22-25 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.4% (W/V) PEG20K |
