2WGT
Structure of human adenovirus serotype 37 fibre head in complex with a sialic acid derivative, O-Methyl 5-N-propaonyl-3,5-dideoxy-D- glycero-a-D-galacto-2-nonulopyranosylonic acid
Summary for 2WGT
| Entry DOI | 10.2210/pdb2wgt/pdb |
| Related | 1UXA 1UXB 1UXE 2J12 2WGU |
| Descriptor | FIBER PROTEIN, 3,5-dideoxy-5-(propanoylamino)-D-glycero-alpha-D-galacto-non-2-ulopyranosonic acid, ZINC ION, ... (4 entities in total) |
| Functional Keywords | sialic acid, viral protein, conjunctivitis, daf, ad37, cd46, receptor, neuraminic acid |
| Biological source | HUMAN ADENOVIRUS 37 |
| Total number of polymer chains | 3 |
| Total formula weight | 66315.72 |
| Authors | Johansson, S.,Nilsson, E.,Qian, W.,Guilligay, D.,Crepin, T.,Cusack, S.,Arnberg, N.,Elofsson, M. (deposition date: 2009-04-27, release date: 2009-11-24, Last modification date: 2023-12-13) |
| Primary citation | Johansson, S.,Nilsson, E.,Qian, W.,Guilligay, D.,Crepin, T.,Cusack, S.,Arnberg, N.,Elofsson, M. Design, Synthesis, and Evaluation of N-Acyl Modified Sialic Acids as Inhibitors of Adenoviruses Causing Epidemic Keratoconjunctivitis. J.Med.Chem., 52:3666-, 2009 Cited by PubMed Abstract: The adenovirus serotype Ad37 binds to and infects human corneal epithelial (HCE) cells through attachment to cellular glycoproteins carrying terminal sialic acids. By use of the crystallographic structure of the sialic acid-interacting domain of the Ad37 fiber protein in complex with sialyllactose, a set of N-acyl modified sialic acids were designed to improve binding affinity through increased hydrophobic interactions. These N-acyl modified sialic acids and their corresponding multivalent human serum albumin (HSA) conjugates were synthesized and tested in Ad37 cell binding and cell infectivity assays. Compounds bearing small substituents were as effective inhibitors as sialic acid. X-ray crystallography and overlays with the Ad37-sialyllactose complex showed that the N-acyl modified sialic acids were positioned in the same orientation as sialic acid. Their multivalent counterparts achieved a strong multivalency effect and were more effective to prevent infection than the monomers. Unfortunately, they were less active as inhibitors than multivalent sialic acid. PubMed: 19456100DOI: 10.1021/JM801609S PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.8 Å) |
Structure validation
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