2WGT
Structure of human adenovirus serotype 37 fibre head in complex with a sialic acid derivative, O-Methyl 5-N-propaonyl-3,5-dideoxy-D- glycero-a-D-galacto-2-nonulopyranosylonic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-16 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.270, 69.990, 75.000 |
Unit cell angles | 90.00, 94.42, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.800 |
R-factor | 0.185 |
Rwork | 0.183 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uxa |
RMSD bond length | 0.010 |
RMSD bond angle | 1.225 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.040 | 0.470 |
Number of reflections | 58545 | |
<I/σ(I)> | 18.4 | 2.88 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 3.74 | 3.73 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 25-27 % POLYETHYLENE GLYCOL 8000, 50 MM ZINC ACETATE, AND 100 MM HEPES PH 7.5 |