2WAH

Crystal Structure of an IgG1 Fc Glycoform (Man9GlcNAc2)

Summary for 2WAH

• Entry DOI: 10.2210/pdb2wah/pdb
• Related: 1AJ7 1AQK 1BEY 1D5B 1D5I 1D6V 1DN2 1E4K 1FC1 1FCC 1H3T 1H3U 1H3V 1H3W 1H3Y 1I7Z 1L6X 1OQX 1T83 1T89 2IWG 2J6E 2RCS
• Descriptor: IG GAMMA-1 CHAIN C REGION, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• Functional Keywords: immunoglobulin c region, antibody engineering, immunoglobulin domain, secreted, antibody, kifunensine, glycoprotein, oligomannose, immune system, glycosylation
• Biological source: Homo sapiens (Human)
• Cellular location: Secreted : P01857
• Total number of polymer chains: 2
• Total formula weight: 49549.46

Authors

Crispin, M.,Bowden, T.A.,Coles, C.H.,Harlos, K.,Aricescu, A.R.,Harvey, D.J.,Stuart, D.I.,Jones, E.Y. (deposition date: 2009-02-06, release date: 2009-03-10, Last modification date: 2023-12-13)

Primary citation

Crispin, M.,Bowden, T.A.,Coles, C.H.,Harlos, K.,Aricescu, A.R.,Harvey, D.J.,Stuart, D.I.,Jones, E.Y.
Carbohydrate and Domain Architecture of an Immature Antibody Glycoform Exhibiting Enhanced Effector Functions
J.Mol.Biol., 387:1061-, 2009

PubMed Abstract: Antibodies contain a conserved glycosylation site that has emerged as a target for the modulation of antibody effector functions. The crystal structure of a biosynthetic intermediate of human IgG1, bearing immature oligomannose-type glycans and reported to display increased antibody-dependent cellular cytotoxicity, demonstrates that glycan engineering can bias the Fc to an open conformation primed for receptor binding.

PubMed: 19236877
DOI: 10.1016/J.JMB.2009.02.033

Experimental method

X-RAY DIFFRACTION (2.51 Å)