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2W9H

Wild-type Staphylococcus aureus DHFR in complex with trimethoprim

Summary for 2W9H
Entry DOI10.2210/pdb2w9h/pdb
Related2W9G
DescriptorDIHYDROFOLATE REDUCTASE, 1,2-ETHANEDIOL, TRIMETHOPRIM, ... (4 entities in total)
Functional Keywordsoxidoreductase, one-carbon metabolism
Biological sourceSTAPHYLOCOCCUS AUREUS
Total number of polymer chains1
Total formula weight18628.32
Authors
Soutter, H.H.,Miller, J.R. (deposition date: 2009-01-23, release date: 2009-03-24, Last modification date: 2024-05-08)
Primary citationHeaslet, H.,Harris, M.,Fahnoe, K.,Sarver, R.,Putz, H.,Chang, J.,Subramanian, C.,Barreiro, G.,Miller, J.R.
Structural Comparison of Chromosomal and Exogenous Dihydrofolate Reductase from Staphylococcus Aureus in Complex with the Potent Inhibitor Trimethoprim.
Proteins, 76:706-, 2009
Cited by
PubMed Abstract: Dihydrofolate reductase (DHFR) is the enzyme responsible for the NADPH-dependent reduction of 5,6-dihydrofolate to 5,6,7,8-tetrahydrofolate, an essential cofactor in the synthesis of purines, thymidylate, methionine, and other key metabolites. Because of its importance in multiple cellular functions, DHFR has been the subject of much research targeting the enzyme with anticancer, antibacterial, and antimicrobial agents. Clinically used compounds targeting DHFR include methotrexate for the treatment of cancer and diaminopyrimidines (DAPs) such as trimethoprim (TMP) for the treatment of bacterial infections. DAP inhibitors of DHFR have been used clinically for >30 years and resistance to these agents has become widespread. Methicillin-resistant Staphylococcus aureus (MRSA), the causative agent of many serious nosocomial and community acquired infections, and other gram-positive organisms can show resistance to DAPs through mutation of the chromosomal gene or acquisition of an alternative DHFR termed "S1 DHFR." To develop new therapies for health threats such as MRSA, it is important to understand the molecular basis of DAP resistance. Here, we report the crystal structure of the wild-type chromosomal DHFR from S. aureus in complex with NADPH and TMP. We have also solved the structure of the exogenous, TMP resistant S1 DHFR, apo and in complex with TMP. The structural and thermodynamic data point to important molecular differences between the two enzymes that lead to dramatically reduced affinity of DAPs to S1 DHFR. These differences in enzyme binding affinity translate into reduced antibacterial activity against strains of S. aureus that express S1 DHFR.
PubMed: 19280600
DOI: 10.1002/PROT.22383
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.48 Å)
Structure validation

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