Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

2LEB

Solution structure of human SRSF2 (SC35) RRM in complex with 5'-UCCAGU-3'

Summary for 2LEB
Entry DOI10.2210/pdb2leb/pdb
Related2LEA 2LEC
NMR InformationBMRB: 17706
DescriptorSerine/arginine-rich splicing factor 2, RNA (5'-R(*UP*CP*CP*AP*GP*U)-3') (2 entities in total)
Functional Keywordssr protein, splicing factor, rna protein complex, rna binding protein-rna complex, rna binding protein/rna
Biological sourceHomo sapiens (human)
More
Cellular locationNucleus: Q01130
Total number of polymer chains2
Total formula weight17110.22
Authors
Daubner, G.M.,Clery, A.,Jayne, S.,Stevenin, J.,Allain, F.H.-T. (deposition date: 2011-06-15, release date: 2011-11-23, Last modification date: 2024-05-08)
Primary citationDaubner, G.M.,Clery, A.,Jayne, S.,Stevenin, J.,Allain, F.H.
A syn-anti conformational difference allows SRSF2 to recognize guanines and cytosines equally well.
Embo J., 31:162-174, 2012
Cited by
PubMed Abstract: SRSF2 (SC35) is a key player in the regulation of alternative splicing events and binds degenerated RNA sequences with similar affinity in nanomolar range. We have determined the solution structure of the SRSF2 RRM bound to the 5'-UCCAGU-3' and 5'-UGGAGU-3' RNA, both identified as SRSF2 binding sites in the HIV-1 tat exon 2. RNA recognition is achieved through a novel sandwich-like structure with both termini forming a positively charged cavity to accommodate the four central nucleotides. To bind both RNA sequences equally well, SRSF2 forms a nearly identical network of intermolecular interactions by simply flipping the bases of the two consecutive C or G nucleotides into either anti or syn conformation. We validate this unusual mode of RNA recognition functionally by in-vitro and in-vivo splicing assays and propose a 5'-SSNG-3' (S=C/G) high-affinity binding consensus sequence for SRSF2. In conclusion, in addition to describe for the first time the RNA recognition mode of SRSF2, we provide the precise consensus sequence to identify new putative binding sites for this splicing factor.
PubMed: 22002536
DOI: 10.1038/emboj.2011.367
PDB entries with the same primary citation
Experimental method
SOLUTION NMR
Structure validation

227561

PDB entries from 2024-11-20

PDB statisticsPDBj update infoContact PDBjnumon