2G9Y

Structure of S102T E. coli alkaline phosphatase in presence of phosphate at 2.00 A resolution

Summary for 2G9Y

• Entry DOI: 10.2210/pdb2g9y/pdb
• Related: 1Y6V 2ga3
• Descriptor: Alkaline phosphatase, ZINC ION, MAGNESIUM ION, ... (6 entities in total)
• Functional Keywords: mutagenesis, side chain conformation, covalent intermediate, rate-determining step, hydrolase
• Biological source: Escherichia coli
• Cellular location: Periplasm: P00634
• Total number of polymer chains: 2
• Total formula weight: 94909.16

Authors

Wang, J.,Kantrowitz, E.R. (deposition date: 2006-03-07, release date: 2006-10-10, Last modification date: 2023-08-30)

• Primary citation: Wang, J.,Kantrowitz, E.R.; Trapping the tetrahedral intermediate in the alkaline phosphatase reaction by substitution of the active site serine with threonine.; Protein Sci., 15:2395-2401, 2006; PubMed: 17008720; DOI: 10.1110/ps.062351506

Experimental method

X-RAY DIFFRACTION (2 Å)