2GA3

Structure of S102T E. coli Alkaline Phosphatase-phosphate intermediate at 2.20A resolution

Summary for 2GA3

Entry DOI	10.2210/pdb2ga3/pdb
Related	2G9Y
Descriptor	Alkaline phosphatase, ZINC ION, MAGNESIUM ION, ... (5 entities in total)
Functional Keywords	mutagenesis, side chain conformation, covalent intermediate, rate-determining step, hydrolase
Biological source	Escherichia coli
Cellular location	Periplasm: P00634
Total number of polymer chains	2
Total formula weight	94879.18
Authors	Wang, J.,Kantrowitz, E.R. (deposition date: 2006-03-07, release date: 2006-10-10, Last modification date: 2023-08-30)
Primary citation	Wang, J.,Kantrowitz, E.R. Trapping the tetrahedral intermediate in the alkaline phosphatase reaction by substitution of the active site serine with threonine. Protein Sci., 15:2395-2401, 2006 Cited by PubMed: 17008720 DOI: 10.1110/ps.062351506 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.2 Å)

Structure validation