2G9Y
Structure of S102T E. coli alkaline phosphatase in presence of phosphate at 2.00 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2003-12-24 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 76.460, 164.490, 192.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.700 - 2.000 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y6v |
RMSD bond length | 0.005 |
RMSD bond angle | 1.330 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.700 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.086 | 0.346 |
Number of reflections | 81030 | |
<I/σ(I)> | 9.2 | |
Completeness [%] | 98.6 | 97.8 |
Redundancy | 3.57 | 3.49 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 2.0M ammonium sulfate, 100mM Tris, 10mM magnesium chloride, 0.01mM zinc chloride, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |