2BIF
6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE H256A MUTANT WITH F6P IN PHOSPHATASE ACTIVE SITE
Summary for 2BIF
Entry DOI | 10.2210/pdb2bif/pdb |
Descriptor | PROTEIN (6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE), octyl beta-D-glucopyranoside, 6-O-phosphono-beta-D-fructofuranose, ... (8 entities in total) |
Functional Keywords | kinase, transferase (phospho), phosphatase, hydrolase (phospho), glycolysis, bifunctional enzyme, transferase, hydrolase |
Biological source | Rattus norvegicus (Norway rat) |
Total number of polymer chains | 2 |
Total formula weight | 110452.00 |
Authors | Yuen, M.H.,Hasemann, C.A. (deposition date: 1998-10-26, release date: 1999-02-16, Last modification date: 2023-08-23) |
Primary citation | Yuen, M.H.,Mizuguchi, H.,Lee, Y.H.,Cook, P.F.,Uyeda, K.,Hasemann, C.A. Crystal structure of the H256A mutant of rat testis fructose-6-phosphate,2-kinase/fructose-2,6-bisphosphatase. Fructose 6-phosphate in the active site leads to mechanisms for both mutant and wild type bisphosphatase activities. J.Biol.Chem., 274:2176-2184, 1999 Cited by PubMed: 9890980DOI: 10.1074/jbc.274.4.2176 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.4 Å) |
Structure validation
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