29HG

Cryo-EM structure of the CUL1-RBX1-SKP1-FBXO22 SCF ubiquition ligase in complex with NSD2 via UNC10088

This is a non-PDB format compatible entry.

Summary for 29HG

• Entry DOI: 10.2210/pdb29hg/pdb
• EMDB information: 57178
• Descriptor: F-box only protein 22, Histone-lysine N-methyltransferase NSD2, S-phase kinase-associated protein 1, ... (6 entities in total)
• Functional Keywords: ubiquitin ligase, targeted protein degradation, cullin-ring ligase, skp1 cul1 f box scf, nuclear receptor binding set domain-containing 2 nsd2, ligase
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 5
• Total formula weight: 318456.73

Authors

Amann, S.J.,Robertson, K.C.,Grishkovskaya, I.,Liu, T.,James, L.I.,Brown, N.B.,Haselbach, D. (deposition date: 2026-03-11, release date: 2026-05-06)

Primary citation

Robertson, K.C.,Amann, S.J.,Liu, T.,Funk, A.V.,Wang, X.,Grishkovskaya, I.,Mehmood, A.,Tabor, J.R.,Norris-Drouin, J.L.,Arrowsmith, C.H.,Collins, J.L.,Miao, Y.,Emanuele, M.J.,Haselbach, D.,James, L.I.,Brown, N.G.
Structural basis of NSD2 degradation via targeted recruitment of SCF-FBXO22.
Nat Commun, 2026

PubMed Abstract: Targeted protein degradation (TPD) through the ubiquitin-proteasome system is driven by compound-mediated polyubiquitination of a protein-of-interest by an E3 ubiquitin (Ub) ligase. Relatively few E3s have been successfully utilized for TPD and the governing principles of functional ternary complex formation between the E3, degrader, and protein target remain elusive. FBXO22 has recently been harnessed for TPD applications by degraders that covalently modify its cysteine residues. Here, we reveal that the aldehyde derivative of UNC10088 promotes cooperative binding of FBXO22 to NSD2, a histone methyltransferase and oncogenic protein, leading to a cryo-EM structure of the SKP1-CUL1-F-box (SCF)-FBXO22 complex with NSD2. This structure revealed a conformational change in the FBXO22 loop surrounding C326, further exposing the cysteine for covalent recruitment. Additional medicinal chemistry efforts led to the discovery of benzaldehyde-based non-prodrug degraders that similarly engage C326 of FBXO22 and potently degrade NSD2. Unlike many degraders, our molecules recruit NSD2 to a different surface of FBXO22 than the known FBXO22 substrate BACH1, allowing for concurrent complex formation and structural determination of SCF bound to both the neosubstrate NSD2 and native substrate BACH1. Overall, we demonstrate the biochemical and structural basis for NSD2 degradation, revealing key principles for efficient and selective TPD by SCF.

PubMed: 42026065
DOI: 10.1038/s41467-026-72235-9

Experimental method

ELECTRON MICROSCOPY (4 Å)