272D

PARALLEL AND ANTIPARALLEL (G.GC)2 TRIPLE HELIX FRAGMENTS IN A CRYSTAL STRUCTURE

Summary for 272D

• Entry DOI: 10.2210/pdb272d/pdb
• Descriptor: DNA (5'-D(*GP*GP*CP*CP*AP*AP*TP*TP*GP*G)-3') (2 entities in total)
• Functional Keywords: b-dna, double helix, intermolecular base triplet, overhanging bases, dna
• Total number of polymer chains: 2
• Total formula weight: 6170.06

Authors

Vlieghe, D.,Van Meervelt, L.,Dautant, A.,Gallois, B.,Precigoux, G.,Kennard, O. (deposition date: 1996-07-09, release date: 1996-09-20, Last modification date: 2024-02-14)

Primary citation

Vlieghe, D.,Van Meervelt, L.,Dautant, A.,Gallois, B.,Precigoux, G.,Kennard, O.
Parallel and antiparallel (G.GC)2 triple helix fragments in a crystal structure.
Science, 273:1702-1705, 1996

PubMed Abstract: Nucleic acid triplexes are formed by sequence-specific interactions between single-stranded polynucleotides and the double helix. These triplexes are implicated in genetic recombination in vivo and have application to areas that include genome analysis and antigene therapy. Despite the importance of the triple helix, only limited high-resolution structural information is available. The x-ray crystal structure of the oligonucleotide d(GGCCAATTGG) is described; it was designed to contain the d(G middle dotGC)2 fragment and thus provide the basic repeat unit of a DNA triple helix. Parameters derived from this crystal structure have made it possible to construct models of both parallel and antiparallel triple helices.

PubMed: 8781231

Experimental method

X-RAY DIFFRACTION (2 Å)