272D
PARALLEL AND ANTIPARALLEL (G.GC)2 TRIPLE HELIX FRAGMENTS IN A CRYSTAL STRUCTURE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE D41A |
| Synchrotron site | LURE |
| Beamline | D41A |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-06-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 26.250, 36.820, 53.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.209 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | IPMOSFLM |
| Data scaling software | ABSCALE |
| Phasing software | AMoRE ((J. NAVAZA)) |
| Refinement software | SHELXL-93 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.500 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.046 * | 0.193 * |
| Total number of observations | 12303 * | |
| Number of reflections | 3534 | |
| <I/σ(I)> | 5.4 | 3.8 |
| Completeness [%] | 94.6 | 86.3 |
| Redundancy | 3.0 * | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 16 * | pH 6.00, VAPOR DIFFUSION, temperature 289.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | SPERMINE | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 0.4 (mM) | |
| 2 | 1 | drop | 43 (mM) | ||
| 3 | 1 | drop | spermine | 0.6 (mM) | |
| 4 | 1 | drop | MPD | 3 (%) | |
| 5 | 1 | reservoir | MPD | 40 (%) |
