26SJ

Crystal structure of MAIT A-F7 TCR-MR1-xanthine

Summary for 26SJ

• Entry DOI: 10.2210/pdb26sj/pdb
• Descriptor: Major histocompatibility complex class I-related gene protein, Beta-2-microglobulin, Human A-F7 TCR TRAV1-2_ALPHA, ... (9 entities in total)
• Functional Keywords: mait cells, mr1, antigen presentation, immune system
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 8
• Total formula weight: 189252.07

Authors

Awad, W.,Rossjohn, J. (deposition date: 2026-05-13, release date: 2026-08-26)

Primary citation

Abdelaal, M.R.,Lim, X.Y.,Mak, J.Y.W.,Fairlie, D.P.,McCluskey, J.,Corbett, A.J.,Gherardin, N.A.,Awad, W.,Rossjohn, J.
The MHC-I related protein 1 MR1 can bind host purine catabolites.
J.Biol.Chem., :113428-113428, 2026

PubMed Abstract: The major histocompatibility complex class I-related molecule (MR1) is a non-classical antigen-presenting protein that presents a diverse array of microbial, synthetic and host-generated small metabolites to mucosal-associated invariant T (MAIT) cells and other MR1-restricted T (MR1T) cell populations. Recent studies showed that MR1 binds carbonyl-nucleobase adducts, resulting in the activation of some MR1T cell clones. However, whether endogenous canonical nucleobases and their derivatives can impact the MR1 axis remains unclear. Here, through biochemical and cellular screening of a library of canonical nucleobases and their catabolites, we demonstrate that the catabolites of purine degradation, namely uric acid, xanthine, and xanthosine, can, at high concentrations, bind MR1 and promote its intracellular retention, resulting in reduced MR1 surface expression. These purine-based nucleobases can compete with microbial and non-microbial ligands for MR1 binding. Analysis of three crystal structures of ligand-bound MR1 shows that these endogenous purine-based nucleobases adopt distinct modes of binding and interactions within the MR1 A'-pocket. Accordingly, we broaden the repertoire of MR1-ligandome by describing a group of host xanthine-based compounds that can bind MR1 and regulate its trafficking to the cell surface.

PubMed: 42586424
DOI: 10.1016/j.jbc.2026.113428

Experimental method

X-RAY DIFFRACTION (2.6 Å)