244D

THE HIGH-RESOLUTION CRYSTAL STRUCTURE OF A PARALLEL-STRANDED GUANINE TETRAPLEX

Summary for 244D

• Entry DOI: 10.2210/pdb244d/pdb
• Descriptor: DNA (5'-D(*TP*GP*GP*GP*GP*T)-3'), SODIUM ION, CALCIUM ION, ... (4 entities in total)
• Functional Keywords: unusual dna, quadruple helix, parallel-stranded tetraplex, dna
• Total number of polymer chains: 16
• Total formula weight: 30766.58

Authors

Laughlan, G.,Murchie, A.I.H.,Norman, D.G.,Moore, M.H.,Moody, P.C.E.,Lilley, D.M.J.,Luisi, B. (deposition date: 1995-10-19, release date: 1996-02-23, Last modification date: 2024-02-14)

Primary citation

Laughlan, G.,Murchie, A.I.,Norman, D.G.,Moore, M.H.,Moody, P.C.,Lilley, D.M.,Luisi, B.
The high-resolution crystal structure of a parallel-stranded guanine tetraplex.
Science, 265:520-524, 1994

PubMed Abstract: Repeat tracts of guanine bases found in DNA and RNA can form tetraplex structures in the presence of a variety of monovalent cations. Evidence suggests that guanine tetraplexes assume important functions within chromosomal telomeres, immunoglobulin switch regions, and the human immunodeficiency virus genome. The structure of a parallel-stranded tetraplex formed by the hexanucleotide d(TG4T) and stabilized by sodium cations was determined by x-ray crystallography to 1.2 angstroms resolution. Sharply resolved sodium cations were found between and within planes of hydrogen-bonded guanine quartets, and an ordered groove hydration was observed. Distinct intra- and intermolecular stacking arrangements were adopted by the guanine quartets. Thymine bases were exclusively involved in making extensive lattice contacts.

PubMed: 8036494

Experimental method

X-RAY DIFFRACTION (1.2 Å)