244D
THE HIGH-RESOLUTION CRYSTAL STRUCTURE OF A PARALLEL-STRANDED GUANINE TETRAPLEX
Experimental procedure
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 1 |
Unit cell lengths | 28.760, 35.470, 56.770 |
Unit cell angles | 74.39, 77.64, 89.73 |
Refinement procedure
Resolution | 8.000 - 1.200 |
R-factor | 0.124 |
R-free | 0.17600 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.600 * |
Data reduction software | DENZO |
Refinement software | SHELX-93 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 3.160 * | |
High resolution limit [Å] | 1.200 | 1.200 * |
Rmerge | 0.056 * | 0.224 * |
Total number of observations | 211674 * | |
Number of reflections | 65264 | 13162 * |
Completeness [%] | 97.7 * | 95.5 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 4 * | pH 6.60, VAPOR DIFFUSION, HANGING DROP, temperature 277.00K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
10 | 1 | 2 | CACL2 | ||
11 | 1 | 2 | SPERMINE | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | NA CACODYLATE | ||
4 | 1 | 1 | CACL2 | ||
5 | 1 | 1 | NACL | ||
6 | 1 | 1 | SPERMINE | ||
7 | 1 | 2 | WATER | ||
8 | 1 | 2 | MPD | ||
9 | 1 | 2 | NA CACODYLATE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Na cacodylate-HCl | 20 (mM) | |
10 | 1 | reservoir | MPD | 32 (%) | |
2 | 1 | drop | 12 (mM) | ||
3 | 1 | drop | spermine | 6 (mM) | |
4 | 1 | drop | 130-180 (mM) | ||
5 | 1 | drop | MPD | 5 (%(v/v)) | |
6 | 1 | drop | TG4T | 1 (mM) | |
7 | 1 | reservoir | sodium cacodylate-HCl | 120 (mM) | |
8 | 1 | reservoir | 70 (mM) | ||
9 | 1 | reservoir | 700-1000 (mM) |