23VW

Cryo-EM structure of the TfCas12m3-crRNA-tgDNA ternary complex

Summary for 23VW

• Entry DOI: 10.2210/pdb23vw/pdb
• EMDB information: 69299
• Descriptor: CRISPR-associated DNA-binding protein Cas12m, DNA (35-MER), DNA (39-MER), ... (7 entities in total)
• Functional Keywords: dna binding protein
• Biological source: Thermanaerosceptrum fracticalcis; More
• Total number of polymer chains: 4
• Total formula weight: 110911.36

Authors

Morinaga, H.,Omura, S.N.,Nureki, O. (deposition date: 2026-02-21, release date: 2026-09-02)

• Primary citation: Morinaga, H.,Omura, S.N.,Alfonse, L.,Ornstein, A.,Kobayashi, T.A.,Onishi, K.,Makarova, K.S.,Shmakov, S.A.,Munoz, G.,Garrity, A.J.,DiTommaso, T.,Koonin, E.V.,Maben, Z.,Nureki, O.; Intact RuvC catalytic site and bipartite ssDNA kinking are required for Cas12m nuclease activation; To Be Published,

Experimental method

ELECTRON MICROSCOPY (2.39 Å)