23VW

Cryo-EM structure of the TfCas12m3-crRNA-tgDNA ternary complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	CRISPR-associated DNA-binding protein Cas12m	polymer	577	67784.8	1	UniProt (A0A7G6E019)	Thermanaerosceptrum fracticalcis	TfCas12m3
2	B (D)	DNA (35-MER)	polymer	39	11905.6	1		DNA molecule
3	C (C)	DNA (39-MER)	polymer	39	12033.7	1		DNA molecule
4	D (B)	RNA (59-MER)	polymer	59	19000.3	1		Thermanaerosceptrum fracticalcis
5	E, F, H, I, J (A, B)	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG) PubChem (888)
6	G (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN) PubChem (32051)
7	K, L, M (A, D, B)	water	water		18.0	18	Chemie (HOH)

Sequence modifications

A: 1 - 567 (UniProt: A0A7G6E019)

PDB	External Database	Details
Met -9	-	initiating methionine
Gly -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	110724.4
Non-Polymers*	Number of molecules	6
	Total formula weight	186.9
All*	Total formula weight	110911.4

*Water molecules are not included.