21EM

Crystal structure of a cupin protein (tm1459, D50N/H52G/H92A/F104W mutant) soaked in CuSO4

Summary for 21EM

• Entry DOI: 10.2210/pdb21em/pdb
• Descriptor: Cupin type-2 domain-containing protein, COPPER (II) ION, PYRIDINE-2-CARBOXYLIC ACID, ... (4 entities in total)
• Functional Keywords: artificial metalloenzymes, biocatalysis, non-heme copper enzymes, metal binding protein
• Biological source: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)
• Total number of polymer chains: 2
• Total formula weight: 27139.35

Authors

Morikawa, S.,Saegusa, N.,Matsumoto, R.,Morita, Y.,Fujieda, N. (deposition date: 2025-12-10, release date: 2026-06-17)

• Primary citation: Morikawa, S.,Saegusa, N.,Matsumoto, R.,Morita, Y.,Fujieda, N.; Structure-Guided Surface Engineering of an Artificial Metallolyase for Highly Enantioselective Michael Addition; Chemcatchem, 18:-, 2026; DOI: 10.1002/cctc.70836

Experimental method

X-RAY DIFFRACTION (1.595 Å)