21EM
Crystal structure of a cupin protein (tm1459, D50N/H52G/H92A/F104W mutant) soaked in CuSO4
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Cupin type-2 domain-containing protein | polymer | 118 | 13349.3 | 2 | UniProt (Q9X1H0) Pfam (PF07883) | Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) | Cupin_2 domain-containing protein |
| 2 | C, D, F, G, H (A, B) | COPPER (II) ION | non-polymer | 63.5 | 5 | Chemie (CU) PubChem (27099) | |||
| 3 | E (A) | PYRIDINE-2-CARBOXYLIC ACID | non-polymer | 123.1 | 1 | Chemie (6PC) PubChem (1018) | |||
| 4 | I, J (A, B) | water | water | 18.0 | 98 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 114 (UniProt: Q9X1H0)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Asn 50 | Asp 50 | engineered mutation |
| Gly 52 | His 52 | engineered mutation |
| Ala 92 | His 92 | engineered mutation |
| Trp 104 | Phe 104 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 26698.5 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 440.8 | |
| All* | Total formula weight | 27139.3 |
