1ZBB

Structure of the 4_601_167 Tetranucleosome

1ZBB の概要

• エントリーDOI: 10.2210/pdb1zbb/pdb
• 関連するPDBエントリー: 1KX5
• 分子名称: DNA STRAND 1 (ARBITRARY MODEL SEQUENCE), DNA STRAND 2 (ARBITRARY MODEL SEQUENCE), HISTONE H3, ... (6 entities in total)
• 機能のキーワード: tetranucleosome, nucleosome, chromatin, chromatin fiber, histone, protein-dna interaction, nucleoprotein, supercoiled dna, nucleosome core, protein-dna complex, structural protein-dna complex, structural protein/dna
• 由来する生物種: Xenopus laevis (African clawed frog); 詳細
• 細胞内の位置: Nucleus: P84233 P62799 P06897 P02281
• タンパク質・核酸の鎖数: 18
• 化学式量合計: 431871.64

構造登録者

Schalch, T.,Duda, S.,Sargent, D.F.,Richmond, T.J. (登録日: 2005-04-08, 公開日: 2005-07-12, 最終更新日: 2024-11-13)

主引用文献

Schalch, T.,Duda, S.,Sargent, D.F.,Richmond, T.J.
X-ray structure of a tetranucleosome and its implications for the chromatin fibre.
Nature, 436:138-141, 2005

PubMed Abstract: DNA in eukaryotic chromosomes is organized in arrays of nucleosomes compacted into chromatin fibres. This higher-order structure of nucleosomes is the substrate for DNA replication, recombination, transcription and repair. Although the structure of the nucleosome core is known at near-atomic resolution, even the most fundamental information about the organization of nucleosomes in the fibre is controversial. Here we report the crystal structure of an oligonucleosome (a compact tetranucleosome) at 9 A resolution, solved by molecular replacement using the nucleosome core structure. The structure shows that linker DNA zigzags back and forth between two stacks of nucleosome cores, which form a truncated two-start helix, and does not follow a path compatible with a one-start solenoidal helix. The length of linker DNA is most probably buffered by stretching of the DNA contained in the nucleosome cores. We have built continuous fibre models by successively stacking tetranucleosomes one on another. The resulting models are nearly fully compacted and most closely resemble the previously described crossed-linker model. They suggest that the interfaces between nucleosomes along a single helix start are polymorphic.

PubMed: 16001076
DOI: 10.1038/nature03686

実験手法

X-RAY DIFFRACTION (9 Å)