1ZBB

Structure of the 4_601_167 Tetranucleosome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2003-08-13
Detector	MARRESEARCH
Wavelength(s)	0.96980
Spacegroup name	I 2 2 2
Unit cell lengths	127.675, 168.445, 237.126
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 9.000
R-factor	0.386
Rwork	0.386
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1KX5(NUCLEOSOME CORE PARTICLE)
Phasing software	BEAST
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	10.000
High resolution limit [Å]	9.000	9.000
Number of reflections	1994
<I/σ(I)>	17.28	12.55
Completeness [%]	97.0	97.4
Redundancy	5.7	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.75	294	magnesium chloride, potassium chloride, potassium cacodylate, trisCl, pH 6.75, VAPOR DIFFUSION, SITTING DROP, temperature 294K
1	VAPOR DIFFUSION, SITTING DROP	6.75	294	magnesium chloride, potassium chloride, potassium cacodylate, trisCl, pH 6.75, VAPOR DIFFUSION, SITTING DROP, temperature 294K
1	VAPOR DIFFUSION, SITTING DROP	6.75	294	magnesium chloride, potassium chloride, potassium cacodylate, trisCl, pH 6.75, VAPOR DIFFUSION, SITTING DROP, temperature 294K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	magnesium chloride
2	1	1	potassium chloride
3	1	1	potassium cacodylate
4	1	1	trisCl
5	1	2	magnesium chloride
6	1	2	potassium chloride
7	1	2	potassium cacodylate
8	1	2	trisCl