1YV6
X-ray structure of M23L onconase at 298K
Summary for 1YV6
| Entry DOI | 10.2210/pdb1yv6/pdb |
| Related | 1YV4 1YV7 |
| Descriptor | P-30 protein, SULFATE ION (3 entities in total) |
| Functional Keywords | small conformational changes, onconase thermal stability, ribonucleases, antitumor action, dynamics, hydrolase |
| Biological source | Rana pipiens (northern leopard frog) |
| Total number of polymer chains | 1 |
| Total formula weight | 11923.67 |
| Authors | Merlino, A.,Mazzarella, L.,Carannante, A.,Di Fiore, A.,Di Donato, A.,Notomista, E.,Sica, F. (deposition date: 2005-02-15, release date: 2005-03-01, Last modification date: 2021-11-10) |
| Primary citation | Merlino, A.,Mazzarella, L.,Carannante, A.,Di Fiore, A.,Di Donato, A.,Notomista, E.,Sica, F. The Importance of Dynamic Effects on the Enzyme Activity: X-RAY STRUCTURE AND MOLECULAR DYNAMICS OF ONCONASE MUTANTS J.Biol.Chem., 280:17953-17960, 2005 Cited by PubMed Abstract: Onconase (ONC), a member of the RNase A superfamily extracted from oocytes of Rana pipiens, is an effective cancer killer. It is currently used in treatment of various forms of cancer. ONC antitumor properties depend on its ribonucleolytic activity that is low in comparison with other members of the superfamily. The most damaging side effect from Onconase treatment is renal toxicity, which seems to be caused by the unusual stability of the enzyme. Therefore, mutants with reduced thermal stability and/or increased catalytic activity may have significant implications for human cancer chemotherapy. In this context, we have determined the crystal structures of two Onconase mutants (M23L-ONC and C87S,des103-104-ONC) and performed molecular dynamic simulations of ONC and C87S,des103-104-ONC with the aim of explaining on structural grounds the modifications of the activity and thermal stability of the mutants. The results also provide the molecular bases to explain the lower catalytic activity of Onconase compared with RNase A and the unusually high thermal stability of the amphibian enzyme. PubMed: 15728177DOI: 10.1074/jbc.M501339200 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.78 Å) |
Structure validation
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