1YTB

CRYSTAL STRUCTURE OF A YEAST TBP/TATA-BOX COMPLEX

Summary for 1YTB

Entry DOI	10.2210/pdb1ytb/pdb
Descriptor	DNA (29MER), PROTEIN (TATA BINDING PROTEIN (TBP)) (3 entities in total)
Functional Keywords	protein-dna complex, transcription-dna complex, transcription/dna
Biological source	Saccharomyces cerevisiae (baker's yeast)
Cellular location	Nucleus: P13393
Total number of polymer chains	4
Total formula weight	58217.14
Authors	Kim, Y.,Geiger, J.H.,Hahn, S.,Sigler, P.B. (deposition date: 1994-09-28, release date: 1995-01-26, Last modification date: 2024-02-14)
Primary citation	Kim, Y.,Geiger, J.H.,Hahn, S.,Sigler, P.B. Crystal structure of a yeast TBP/TATA-box complex. Nature, 365:512-520, 1993 Cited by PubMed Abstract: The 2.5 A crystal structure of a TATA-box complex with yeast TBP shows that the eight base pairs of the TATA box bind to the concave surface of TBP by bending towards the major groove with unprecedented severity. This produces a wide open, underwound, shallow minor groove which forms a primarily hydrophobic interface with the entire under-surface of the TBP saddle. The severe bend and a positive writhe radically alter the trajectory of the flanking B-form DNA. PubMed: 8413604 DOI: 10.1038/365512a0 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.8 Å)

Structure validation