1YTB
CRYSTAL STRUCTURE OF A YEAST TBP/TATA-BOX COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Detector technology | AREA DETECTOR |
| Detector | XENTRONICS |
| Spacegroup name | P 43 |
| Unit cell lengths | 100.770, 100.770, 66.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.800 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.26100 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.560 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Number of reflections | 59705 |
| Completeness [%] | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 22 * | pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 295.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 10 | 1 | 2 | ETHYLENE GLYCOL | ||
| 2 | 1 | 1 | PEG 8000 | ||
| 3 | 1 | 1 | GLYCEROL | ||
| 4 | 1 | 1 | ETHYLENE GLYCOL | ||
| 5 | 1 | 1 | NACL | ||
| 6 | 1 | 1 | BIS-TRIS-PROPANE_HCL | ||
| 7 | 1 | 2 | WATER | ||
| 8 | 1 | 2 | PEG 8000 | ||
| 9 | 1 | 2 | GLYCEROL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.25 (mM) | |
| 10 | 1 | reservoir | ethylene glycol | 2 (%) | |
| 11 | 1 | reservoir | 500 (mM) | ||
| 2 | 1 | drop | DNA | 0.5 (mM) | |
| 3 | 1 | drop | 500 (mM) | ||
| 4 | 1 | drop | 1,3-bis(tris-(hydroxymethyl)-methylamino) propane | 20 (mM) | |
| 5 | 1 | drop | PEG8000 | 15 (%) | |
| 6 | 1 | drop | ethylene glycol | 1 (%) | |
| 7 | 1 | reservoir | PEG8000 | 30 (%) | |
| 8 | 1 | reservoir | 1,3-bis(tris-(hydroxymethyl)-methylamino) propane | 40 (mM) | |
| 9 | 1 | reservoir | glycerol | 2.5 (%) |
