1YBK
RHCC cocrystallized with CAPB
Summary for 1YBK
| Entry DOI | 10.2210/pdb1ybk/pdb |
| Related | 1fe6 |
| Descriptor | tetrabrachion, SULFATE ION, N-(CARBOXYMETHYL)-N,N-DIMETHYL-3-[(1-OXODODECYL)AMINO]-1-PROPANAMINIUM INNER SALT, ... (4 entities in total) |
| Functional Keywords | right hand coiled coil, protein binding |
| Biological source | Staphylothermus marinus |
| Total number of polymer chains | 4 |
| Total formula weight | 24225.59 |
| Authors | Stetefeld, J. (deposition date: 2004-12-21, release date: 2005-01-18, Last modification date: 2024-02-14) |
| Primary citation | Ozbek, S.,Muller, J.F.,Figgemeier, E.,Stetefeld, J. Favourable mediation of crystal contacts by cocoamidopropylbetaine (CAPB). Acta Crystallogr.,Sect.D, 61:477-480, 2005 Cited by PubMed Abstract: Crystals of excellent quality are a prerequisite for high-resolution X-ray data. However, in refinement protocols of crystallization conditions it is often difficult to obtain the right combination of, for example, protein concentration, drop size, temperature and additives. A novel approach for optimizing crystal contacts in a most favourable fashion by performing crystallization setups with the zwitterionic surfactant cocoamidoproylbetaine (CAPB) is introduced. In the presence of this surfactant, highly diffracting crystals were obtained. Here, data from a right-handed coiled coil (RHCC) in complex with CAPB at 1.4 A resolution are presented. The addition of CAPB using otherwise identical crystallization conditions and the same X-ray source caused an improvement in resolution from 2.9 to 1.4 A. PubMed: 15805603DOI: 10.1107/S0907444905001204 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.45 Å) |
Structure validation
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