1Y5M

The crystal structure of murine 11b-hydroxysteroid dehydrogenase: an important therapeutic target for diabetes

1Y5M の概要

• エントリーDOI: 10.2210/pdb1y5m/pdb
• 関連するPDBエントリー: 1Y5R
• 分子名称: Corticosteroid 11-beta-dehydrogenase, isozyme 1, SULFATE ION, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, ... (5 entities in total)
• 機能のキーワード: rossmann fold, oxidoreductase
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Endoplasmic reticulum membrane; Single-pass type II membrane protein: P50172
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 63274.91

構造登録者

Zhang, J.,Osslund, T.D.,Plant, M.H.,Clogston, C.L.,Nybo, R.E.,Xiong, F.,Delaney, J.M.,Jordan, S.R. (登録日: 2004-12-02, 公開日: 2005-05-17, 最終更新日: 2024-02-14)

主引用文献

Zhang, J.,Osslund, T.D.,Plant, M.H.,Clogston, C.L.,Nybo, R.E.,Xiong, F.,Delaney, J.M.,Jordan, S.R.
Crystal Structure of Murine 11-Hydroxysteroid Dehydrogenase 1: An Important Therapeutic Target for Diabetes
Biochemistry, 44:6948-6957, 2005

PubMed Abstract: 11Beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) catalyzes the conversion of 11-dehydrocorticosterone to its active form corticosterone in rodents (or cortisone to cortisol in humans). The reductive reaction of the 11-keto to 11-hydroxyl is the pivotal switch in the activation of glucocorticoids. An excess of active glucocorticoids has been shown to play a key role in metabolic disorders such as diabetes and obesity. Therefore, 11beta-HSD1 represents an important therapeutic target for the treatment of these diseases. To facilitate the iterative design of inhibitors, we have crystallized and determined the three-dimensional structures of a binary complex of murine 11beta-HSD1 with NADP(H) to a resolution of 2.3 A and of a ternary complex with corticosterone and NADP(H) to a resolution of 3.0 A by X-ray crystallography. The enzyme forms a homodimer in the crystal and has a fold similar to those of other members of the family of short chain steroid dehydrogenases/reductases (SDRs). The structure shows a novel folding feature at the C-terminus of the enzyme. The C-terminal helix insertions provide additional dimer contacts, exert an influence on the conformations of the substrate binding loops, and present hydrophobic regions for potential membrane attachment. The structure also reveals how 11beta-HSD1 achieves its selectivity for its substrate.

PubMed: 15865440
DOI: 10.1021/bi047599q

実験手法

X-RAY DIFFRACTION (2.3 Å)