1Y5M
The crystal structure of murine 11b-hydroxysteroid dehydrogenase: an important therapeutic target for diabetes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 96.718, 96.718, 219.302 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.300 |
| Rwork | 0.219 |
| R-free | 0.25000 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.083 |
| RMSD bond angle | 1.786 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.260 |
| Rmerge | 0.074 | 0.450 |
| Number of reflections | 43963 | |
| <I/σ(I)> | 17.5 | 2.2 |
| Completeness [%] | 93.0 | 91 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 1.8 M Li2SO4, and 0.1 M Hepes buffer, pH 7.5 , VAPOR DIFFUSION, temperature 293K |
