1Y5M

The crystal structure of murine 11b-hydroxysteroid dehydrogenase: an important therapeutic target for diabetes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-13
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 41 2 2
Unit cell lengths	96.718, 96.718, 219.302
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.300
Rwork	0.219
R-free	0.25000
Structure solution method	SIRAS
RMSD bond length	0.083
RMSD bond angle	1.786
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.340
High resolution limit [Å]	2.300	2.260
Rmerge	0.074	0.450
Number of reflections	43963
<I/σ(I)>	17.5	2.2
Completeness [%]	93.0	91
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	1.8 M Li2SO4, and 0.1 M Hepes buffer, pH 7.5 , VAPOR DIFFUSION, temperature 293K