1Y54

Crystal structure of the native class C beta-lactamase from Enterobacter cloacae 908R complexed with BRL42715

Summary for 1Y54

Entry DOI	10.2210/pdb1y54/pdb
Descriptor	Beta-lactamase, (7R)-6-FORMYL-7-(1-METHYL-1H-1,2,3-TRIAZOL-4-YL)-4,7-DIHYDRO-1,4-THIAZEPINE-3-CARBOXYLIC ACID (3 entities in total)
Functional Keywords	class c beta-lactamase, hydrolase
Biological source	Enterobacter cloacae
Cellular location	Periplasm : P05364
Total number of polymer chains	1
Total formula weight	39576.09
Authors	Michaux, C.,Charlier, P.,Frere, J.-M.,Wouters, J. (deposition date: 2004-12-02, release date: 2005-03-29, Last modification date: 2024-11-13)
Primary citation	Michaux, C.,Charlier, P.,Frere, J.-M.,Wouters, J. Crystal Structure of BRL 42715, C6-(N1-Methyl-1,2,3-triazolylmethylene)penem, in Complex with Enterobactercloacae 908R beta-Lactamase: Evidence for a Stereoselective Mechanism from Docking Studies J.Am.Chem.Soc., 127:3262-3263, 2005 Cited by PubMed Abstract: BRL 42715, C6-(N1-methyl-1,2,3-triazolylmethylene)penem, is an active-site-directed inactivator of bacterial beta-lactamases. The crystal structure of Enterobacter cloacae 908R class C beta-lactamase in complex with BRL 42715, docking, and energy minimization studies explain stereoselectivity of the binding of C6-(heterocyclic methylene)penems against class C beta-lactamase. PubMed: 15755127 DOI: 10.1021/ja0426241 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.1 Å)

Structure validation