1Y54
Crystal structure of the native class C beta-lactamase from Enterobacter cloacae 908R complexed with BRL42715
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE |
Synchrotron site | LURE |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-09-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.966 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 44.365, 82.221, 96.156 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.100 |
R-factor | 0.227 |
Rwork | 0.227 |
R-free | 0.29190 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.021 |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | |
High resolution limit [Å] | 2.070 | 2.070 |
Number of reflections | 20669 | |
Completeness [%] | 90.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | PEG 6000 30%, bicine 100mM, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |