1XXV

Yersinia YopH (residues 163-468) binds phosphonodifluoromethyl-Phe containing hexapeptide at two sites

Summary for 1XXV

• Entry DOI: 10.2210/pdb1xxv/pdb
• Related: 1QZ0 1XXP
• Descriptor: Protein-tyrosine phosphatase yopH, Epidermal growth factor receptor derived peptide (3 entities in total)
• Functional Keywords: catalytic domain, phosphotyrosine-binding sites, substrate targeting, hydrolase
• Biological source: Yersinia enterocolitica; More
• Cellular location: Secreted: P15273
• Total number of polymer chains: 6
• Total formula weight: 70490.60

Authors

Ivanov, M.I.,Stuckey, J.A.,Schubert, H.L.,Saper, M.A.,Bliska, J.B. (deposition date: 2004-11-08, release date: 2005-03-22, Last modification date: 2024-11-20)

Primary citation

Ivanov, M.I.,Stuckey, J.A.,Schubert, H.L.,Saper, M.A.,Bliska, J.B.
Two substrate-targeting sites in the Yersinia protein tyrosine phosphatase co-operate to promote bacterial virulence
Mol.Microbiol., 55:1346-1356, 2005

PubMed Abstract: YopH is a protein tyrosine phosphatase and an essential virulence determinant of the pathogenic bacterium Yersinia. Yersinia delivers YopH into infected host cells using a type III secretion mechanism. YopH dephosphorylates several focal adhesion proteins including p130Cas in human epithelial cells, resulting in disruption of focal adhesions and cell detachment from the extracellular matrix. How the C-terminal protein tyrosine phosphatase domain of YopH targets specific substrates such as p130Cas in the complex milieu of the host cell has not been fully elucidated. An N-terminal non-catalytic domain of YopH binds p130Cas in a phosphotyrosine-dependent manner and functions as a novel substrate-targeting site. The structure of the YopH protein tyrosine phosphatase domain bound to a model phosphopeptide substrate was solved and the resulting structure revealed a second substrate-targeting site ('site 2') within the catalytic domain. Site 2 binds to p130Cas in a phosphotyrosine-dependent manner, and co-operates with the N-terminal domain ('site 1') to promote efficient recognition of p130Cas by YopH in epithelial cells. The identification of two substrate-targeting sites in YopH that co-operate to promote epithelial cell detachment and bacterial virulence reinforces the importance of protein-protein interactions for determining protein tyrosine phosphatase specificity in vivo, and highlights the sophisticated nature of microbial pathogenicity factors.

PubMed: 15720545
DOI: 10.1111/j.1365-2958.2005.04477.x

Experimental method

X-RAY DIFFRACTION (2.5 Å)