1XMO

Crystal Structure of mnm5U34t6A37-tRNALysUUU Complexed with AAG-mRNA in the Decoding Center

1XMO の概要

• エントリーDOI: 10.2210/pdb1xmo/pdb
• 関連するPDBエントリー: 1FKA 1IBK 1IBL 1IBM 1XMQ 1XNQ 1XNR
• 分子名称: 16S ribosomal RNA, 30S ribosomal protein S8, 30S ribosomal protein S9, ... (26 entities in total)
• 機能のキーワード: ribosome, decoding, trna modification
• 由来する生物種: Thermus thermophilus; 詳細
• タンパク質・核酸の鎖数: 23
• 化学式量合計: 791605.01

構造登録者

Murphy, F.V.,Ramakrishnan, V.,Malkiewicz, A.,Agris, P.F. (登録日: 2004-10-04, 公開日: 2004-12-14, 最終更新日: 2024-10-09)

主引用文献

Murphy, F.V.,Ramakrishnan, V.,Malkiewicz, A.,Agris, P.F.
The role of modifications in codon discrimination by tRNA(Lys)(UUU).
Nat.Struct.Mol.Biol., 11:1186-1191, 2004

PubMed Abstract: The natural modification of specific nucleosides in many tRNAs is essential during decoding of mRNA by the ribosome. For example, tRNA(Lys)(UUU) requires the modification N6-threonylcarbamoyladenosine at position 37 (t(6)A37), adjacent and 3' to the anticodon, to bind AAA in the A site of the ribosomal 30S subunit. Moreover, it can only bind both AAA and AAG lysine codons when doubly modified with t(6)A37 and either 5-methylaminomethyluridine or 2-thiouridine at the wobble position (mnm(5)U34 or s(2)U34). Here we report crystal structures of modified tRNA anticodon stem-loops bound to the 30S ribosomal subunit with lysine codons in the A site. These structures allow the rationalization of how modifications in the anticodon loop enable decoding of both lysine codons AAA and AAG.

PubMed: 15558052
DOI: 10.1038/nsmb861

実験手法

X-RAY DIFFRACTION (3.25 Å)