1X2J

Structural basis for the defects of human lung cancer somatic mutations in the repression activity of Keap1 on Nrf2

1X2J の概要

• エントリーDOI: 10.2210/pdb1x2j/pdb
• 関連するPDBエントリー: 1X2R
• 分子名称: Kelch-like ECH-associated protein 1, SULFATE ION (3 entities in total)
• 機能のキーワード: beta propeller, structural genomics, nppsfa, national project on protein structural and functional analyses, riken structural genomics/proteomics initiative, rsgi, transcription
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Cytoplasm: Q9Z2X8
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35480.41

構造登録者

Padmanabhan, B.,Tong, K.I.,Nakamura, Y.,Ohta, T.,Scharlock, M.,Kobayashi, A.,Ohtsuji, M.,Kang, M.-I.,Yamamoto, M.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 2005-04-25, 公開日: 2006-03-07, 最終更新日: 2024-03-13)

主引用文献

Padmanabhan, B.,Tong, K.I.,Ohta, T.,Nakamura, Y.,Scharlock, M.,Ohtsuji, M.,Kang, M.-I.,Kobayashi, A.,Yokoyama, S.,Yamamoto, M.
Structural basis for defects of keap1 activity provoked by its point mutations in lung cancer
Mol.Cell, 21:689-700, 2006

PubMed Abstract: Nrf2 regulates the cellular oxidative stress response, whereas Keap1 represses Nrf2 through its molecular interaction. To elucidate the molecular mechanism of the Keap1 and Nrf2 interaction, we resolved the six-bladed beta propeller crystal structure of the Kelch/DGR and CTR domains of mouse Keap1 and revealed that extensive inter- and intrablade hydrogen bonds maintain the structural integrity and proper association of Keap1 with Nrf2. A peptide containing the ETGE motif of Nrf2 binds the beta propeller of Keap1 at the entrance of the central cavity on the bottom side via electrostatic interactions with conserved arginine residues. We found a somatic mutation and a gene variation in human lung cancer cells that change glycine to cysteine in the DGR domain, introducing local conformational changes that reduce Keap1's affinity for Nrf2. These results provide a structural basis for the loss of Keap1 function and gain of Nrf2 function.

PubMed: 16507366
DOI: 10.1016/j.molcel.2006.01.013

実験手法

X-RAY DIFFRACTION (1.6 Å)