1W63

AP1 clathrin adaptor core

1W63 の概要

• エントリーDOI: 10.2210/pdb1w63/pdb
• 関連するPDBエントリー: 1GYU 1GYV 1GYW
• 分子名称: ADAPTER-RELATED PROTEIN COMPLEX 1 GAMMA 1 SUBUNIT, ADAPTER-RELATED PROTEIN COMPLEX 1 BETA 1 SUBUNIT, ADAPTOR-RELATED PROTEIN COMPLEX 1, MU 1 SUBUNIT, ... (4 entities in total)
• 機能のキーワード: endocytosis, clathrin adaptor, transport, coated pits
• 由来する生物種: MUS MUSCULUS (MOUSE); 詳細
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 1218983.42

構造登録者

Heldwein, E.,Macia, E.,Wang, J.,Yin, H.L.,Kirchhausen, T.,Harrison, S.C. (登録日: 2004-08-12, 公開日: 2004-09-21, 最終更新日: 2023-12-13)

主引用文献

Heldwein, E.,Macia, E.,Wang, J.,Yin, H.L.,Kirchhausen, T.,Harrison, S.C.
Crystal Structure of the Clathrin Adaptor Protein 1 Core
Proc.Natl.Acad.Sci.USA, 101:14108-, 2004

PubMed Abstract: The heterotetrameric adaptor proteins (AP complexes) link the outer lattice of clathrin-coated vesicles with membrane-anchored cargo molecules. We report the crystal structure of the core of the AP-1 complex, which functions in the trans-Golgi network (TGN). Packing of complexes in the crystal generates an exceptionally long (1,135-A) unit-cell axis, but the 6-fold noncrystallographic redundancy yields an excellent map at 4-A resolution. The AP-1 core comprises N-terminal fragments of the two large chains, beta1 and gamma, and the intact medium and small chains, micro1 and sigma1. Its molecular architecture closely resembles that of the core of AP-2, the plasma-membrane-specific adaptor, for which a structure has been determined. Both structures represent an "inactive" conformation with respect to binding of cargo with a tyrosine-based sorting signal. TGN localization of AP-1 depends on the small GTPase, Arf1, and the phosphoinositide, PI-4-P. We show that directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells and diminish PI-4-P-dependent, but not Arf1-dependent, liposome binding in vitro.

PubMed: 15377783
DOI: 10.1073/PNAS.0406102101

実験手法

X-RAY DIFFRACTION (4 Å)