1VYO

Crystal structure of avidin

Summary for 1VYO

• Entry DOI: 10.2210/pdb1vyo/pdb
• Related: 1AVD 1AVE 1IJ8 1LDO 1LDQ 1LEL 1NQN 1RAV 2AVI 2CAM
• Descriptor: AVIDIN, GLYCEROL (3 entities in total)
• Functional Keywords: glycoprotein, biotin
• Biological source: GALLUS GALLUS (CHICKEN)
• Total number of polymer chains: 2
• Total formula weight: 29274.86

Authors

Airenne, T.T.,Johnson, M.S.,Salminen, T.A. (deposition date: 2004-05-03, release date: 2005-07-20, Last modification date: 2024-11-20)

Primary citation

Repo, S.,Paldanius, T.A.,Hytonen, V.P.,Nyholm, T.K.,Halling, K.K.,Huuskonen, J.,Pentikainen, O.T.,Rissanen, K.,Slotte, J.P.,Airenne, T.T.,Salminen, T.A.,Kulomaa, M.S.,Johnson, M.S.
Binding Properties of Haba-Type Azo Derivatives to Avidin and Avidin-Related Protein 4.
Chem.Biol., 13:1029-, 2006

PubMed Abstract: The chicken genome encodes several biotin-binding proteins, including avidin and avidin-related protein 4 (AVR4). In addition to D-biotin, avidin binds an azo dye compound, 4-hydroxyazobenzene-2-carboxylic acid (HABA), but the HABA-binding properties of AVR4 are not yet known. Differential scanning calorimetry, UV/visible spectroscopy, and molecular modeling were used to analyze the binding of 15 azo molecules to avidin and AVR4. Significant differences are seen in azo compound preferences for the two proteins, emphasizing the importance of the loop between strands beta3 and beta4 for azo ligand recognition; information on these loops is provided by the high-resolution (1.5 A) X-ray structure for avidin reported here. These results may be valuable in designing improved tools for avidin-based life science and nanobiotechnology applications.

PubMed: 17052607
DOI: 10.1016/J.CHEMBIOL.2006.08.006

Experimental method

X-RAY DIFFRACTION (1.48 Å)