1VY7

Crystal structure of the Thermus thermophilus 70S ribosome in the pre-attack state of peptide bond formation containing short substrate-mimic Cytidine-Cytidine-Puromycin in the A site and acylated tRNA in the P site.

This is a large structure.

Summary for 1VY7

Integrates4QCY 4QCZ 4QD0 4QD1
Descriptor16S Ribosomal RNA, 30S ribosomal protein S2, 30S ribosomal protein S3, ... (61 entities in total)
Functional Keywords70s, ribosome, translation, peptydil transfer reaction, proton transfer mechanism, acylated trna, cytidine-cytidine-puromycin
Biological sourceEscherichia coli
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Total number of polymer chains112
Total molecular weight4501050.42
Authors
Polikanov, Y.S.,Steitz, T.A.,Innis, C.A. (deposition date: 2014-05-13, release date: 2014-08-20, Last modification date: 2015-01-28)
Primary citation
Polikanov, Y.S.,Steitz, T.A.,Innis, C.A.
A proton wire to couple aminoacyl-tRNA accommodation and peptide-bond formation on the ribosome.
Nat.Struct.Mol.Biol., 21:787-793, 2014
PubMed: 25132179 (PDB entries with the same primary citation)
DOI: 10.1038/nsmb.2871
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.8 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone0.281141.0%9.5%6.3%0.47MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 1vy7
no rotation
Molmil generated image of 1vy7
rotated about x axis by 90°
Molmil generated image of 1vy7
rotated about y axis by 90°