1VY7

Crystal structure of the Thermus thermophilus 70S ribosome in the pre-attack state of peptide bond formation containing short substrate-mimic Cytidine-Cytidine-Puromycin in the A site and acylated tRNA in the P site.

This is a large structure.

Refinement Statistics

Experimental method:X-RAY DIFFRACTION (2.8 Å)

Cell axes207.560444.230613.030
Cell angles90.0090.0090.00
SpacegroupP 21 21 21
Resolution limits150.38 - 2.80
the highest resolution shell value2.832 - 2.800
R-factor0.236
R-work0.23360
the highest resolution shell value0.348
R-free0.28030
the highest resolution shell value0.394
RMSD bond length0.005
RMSD bond angle0.991

Data Collection Statistics

Resolution limits222.12 - 2.80
the highest resolution shell value -
Number of reflections1345662
Rmerge_l_obs0.184
the highest resolution shell value0.013
Completeness98.0
Redundancy3.79
I/sigma(I)-3

Crystallization Conditions

crystal IDmethodpHpH rangetemperatureunit
1VAPOR DIFFUSION7.6292

Crystallization Reagents

IDcrystal IDsolution IDreagent nameconcentrationdetails
Crystallization Reagents in Literatures*
IDcrystal IDsolutionreagent nameconcentration (unit)details
Annotated Information is extracted from Literature Info*