1VRQ

Crystal Structure of Heterotetrameric Sarcosine Oxidase from Corynebacterium sp. U-96 in complex with Folinic Acid

Summary for 1VRQ

• Entry DOI: 10.2210/pdb1vrq/pdb
• Related: 1EL5 1L9F 1PJ5 1PJ6 1PJ7 1WOO 1X31
• Descriptor: Sarcosine oxidase alpha subunit, N,N-DIMETHYLGLYCINE, ZINC ION, ... (12 entities in total)
• Functional Keywords: heterotetrameric sarcosine oxidase, flavoenzyme, oxidoreductase
• Biological source: Corynebacterium sp.; More
• Total number of polymer chains: 4
• Total formula weight: 183873.37

Authors

Ida, K.,Moriguchi, T.,Suzuki, H. (deposition date: 2005-04-27, release date: 2005-07-05, Last modification date: 2023-12-27)

Primary citation

Ida, K.,Moriguchi, T.,Suzuki, H.
Crystal structure of heterotetrameric sarcosine oxidase from Corynebacterium sp. U-96
BIOCHEM.BIOPHYS.RES.COMMUN., 333:359-366, 2005

PubMed Abstract: Sarcosine oxidase from Corynebacterium sp. U-96 is a heterotetrameric enzyme. Here we report the crystal structures of the enzyme in complex with dimethylglycine and folinic acid. The alpha subunit is composed of two domains, contains NAD(+), and binds folinic acid. The beta subunit contains dimethylglycine, FAD, and FMN, and these flavins are approximately 10A apart. The gamma subunit is in contact with two domains of alpha subunit and has possibly a folate-binding structure. The delta subunit contains a single atom of zinc and has a Cys(3)His zinc finger structure. Based on the structures determined and on the previous works, the structure-function relationship on the heterotetrameric sarcosine oxidase is discussed.

PubMed: 15946648
DOI: 10.1016/j.bbrc.2005.05.116

Experimental method

X-RAY DIFFRACTION (2.2 Å)