1VKJ

Crystal structure of heparan sulfate 3-O-sulfotransferase isoform 1 in the presence of PAP

「1S6T」から置き換えられました

1VKJ の概要

• エントリーDOI: 10.2210/pdb1vkj/pdb
• 関連するPDBエントリー: 1NST
• 分子名称: heparan sulfate (glucosamine) 3-O-sulfotransferase 1, SULFATE ION, ADENOSINE-3'-5'-DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: sulfotransferase, heparan sulfate, pap, papss, transferase
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Golgi apparatus lumen : O35310
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 101265.74

構造登録者

Thorp, S.,Lee, K.A.,Negishi, M.,Linhardt, R.J.,Liu, J.,Pedersen, L.C. (登録日: 2004-05-25, 公開日: 2004-06-01, 最終更新日: 2024-11-13)

主引用文献

Edavettal, S.C.,Lee, K.A.,Negishi, M.,Linhardt, R.J.,Liu, J.,Pedersen, L.C.
Crystal structure and mutational analysis of heparan sulfate 3-O-sulfotransferase isoform 1
J.Biol.Chem., 279:25789-25797, 2004

PubMed Abstract: Heparan sulfate interacts with antithrombin, a protease inhibitor, to regulate blood coagulation. Heparan sulfate 3-O-sulfotransferase isoform 1 performs the crucial last step modification in the biosynthesis of anticoagulant heparan sulfate. This enzyme transfers the sulfuryl group (SO(3)) from 3'-phosphoadenosine 5'-phosphosulfate to the 3-OH position of a glucosamine residue to form the 3-O-sulfo glucosamine, a structural motif critical for binding of heparan sulfate to antithrombin. In this study, we report the crystal structure of 3-O-sulfotransferase isoform 1 at 2.5-A resolution in a binary complex with 3'-phosphoadenosine 5'-phosphate. This structure reveals residues critical for 3'-phosphoadenosine 5'-phosphosulfate binding and suggests residues required for the binding of heparan sulfate. In addition, site-directed mutagenesis analyses suggest that residues Arg-67, Lys-68, Arg-72, Glu-90, His-92, Asp-95, Lys-123, and Arg-276 are essential for enzymatic activity. Among these essential amino acid residues, we find that residues Arg-67, Arg-72, His-92, and Asp-95 are conserved in heparan sulfate 3-O-sulfotransferases but not in heparan N-deacetylase/N-sulfotransferase, suggesting a role for these residues in conferring substrate specificity. Results from this study provide information essential for understanding the biosynthesis of anticoagulant heparan sulfate and the general mechanism of action of heparan sulfate sulfotransferases.

PubMed: 15060080
DOI: 10.1074/jbc.M401089200

実験手法

X-RAY DIFFRACTION (2.5 Å)