1UCB

STRUCTURE OF UNCOMPLEXED FAB COMPARED TO COMPLEX (1CLY, 1CLZ)

Summary for 1UCB

• Entry DOI: 10.2210/pdb1ucb/pdb
• Descriptor: CHIMERIC HUMAN/MOUSE IGG FAB FRAGMENT BR96, SULFATE ION, ... (4 entities in total)
• Functional Keywords: fab, immunoglobulin, antibody, anti-tumor, chimera
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 2
• Total formula weight: 47783.40

Authors

Sheriff, S.,Bajorath, J. (deposition date: 1996-02-06, release date: 1997-03-12, Last modification date: 2024-11-20)

Primary citation

Sheriff, S.,Chang, C.Y.,Jeffrey, P.D.,Bajorath, J.
X-ray structure of the uncomplexed anti-tumor antibody BR96 and comparison with its antigen-bound form.
J.Mol.Biol., 259:938-946, 1996

PubMed Abstract: The X-ray structure of the uncomplexed human chimeric Fab' of the anti-tumor antibody BR96 has been determined at 2.6 A resolution. The structure has been compared with Lewis Y antigen-complexed structures of BR96 which were determined previously. The comparison reveals segmental motions and/or conformational rearrangements of three CDR loops (L1, L3, and H2), whereas CDR H3 does not undergo changes upon complexation despite its significant main-chain contacts to the carbohydrate antigen. In light of the uncomplexed chimeric Fab' structure reported here, the previously observed high mobility of the CL:CH1 domains of the complexed chimeric BR96 Fab is rationalized as a "swinging" motion approximately about the axis of the elbow bend.

PubMed: 8683596
DOI: 10.1006/jmbi.1996.0371

Experimental method

X-RAY DIFFRACTION (2.5 Å)