1UCB
STRUCTURE OF UNCOMPLEXED FAB COMPARED TO COMPLEX (1CLY, 1CLZ)
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 295 |
Detector technology | AREA DETECTOR |
Collection date | 1993-03 |
Detector | SIEMENS-NICOLET X100 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 61.100, 174.300, 45.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.500 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.27600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | FV OF 1CLZ WITH CDRS REMOVED 1CLY (HUMAN IGG1 HUMAN KAPPA) AND OF 1CLZ (MOUSE IGG3 MOUSE KAPPA) |
RMSD bond length | 0.009 |
RMSD bond angle | 28.000 * |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MERLOT |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.000 | 2.800 |
High resolution limit [Å] | 2.500 | 2.530 * |
Rmerge | 0.045 | 0.107 |
Total number of observations | 29571 * | |
Number of reflections | 14826 | |
<I/σ(I)> | 16.1 | 4 |
Completeness [%] | 87.0 | 65 |
Redundancy | 2 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 7 * | 0.1 M SODIUM ACETATE (PH 4.6), 0.1 M AMMONIUM SULFATE, 15 % PEG 8000. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 8 (mg/ml) | |
2 | 1 | 2 | imidazole-HCl | 10 (mM) |