1U5W

Crystal structure of hypothetical protein yjjX from Escherichia coli

1U5W の概要

• エントリーDOI: 10.2210/pdb1u5w/pdb
• 分子名称: Hypothetical UPF0244 protein yjjX, SULFATE ION (3 entities in total)
• 機能のキーワード: 3 layers alpha/beta/alpha protein, montreal-kingston bacterial structural genomics initiative, bsgi, structural genomics, unknown function
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 161029.47

構造登録者

Zheng, J.,Singh, V.K.,Jia, Z.,Montreal-Kingston Bacterial Structural Genomics Initiative (BSGI) (登録日: 2004-07-28, 公開日: 2005-08-23, 最終更新日: 2024-02-14)

主引用文献

Zheng, J.,Singh, V.K.,Jia, Z.
Identification of an ITPase/XTPase in Escherichia coli by Structural and Biochemical Analysis
Structure, 13:1511-1520, 2005

PubMed Abstract: Inosine triphosphate (ITP) and xanthosine triphosphate (XTP) are formed upon deamination of ATP and GTP as a result of exposure to chemical mutagens and oxidative damage. Nucleic acid synthesis requires safeguard mechanisms to minimize undesired lethal incorporation of ITP and XTP. Here, we present the crystal structure of YjjX, a protein of hitherto unknown function. The three-dimensional fold of YjjX is similar to those of Mj0226 from Methanococcus janschii, which possesses nucleotidase activity, and of Maf from Bacillus subtilis, which can bind nucleotides. Biochemical analyses of YjjX revealed it to exhibit specific phosphatase activity for inosine and xanthosine triphosphates and have a possible interaction with elongation factor Tu. The enzymatic activity of YjjX as an inosine/xanthosine triphosphatase provides evidence for a plausible protection mechanism by clearing the noncanonical nucleotides from the cell during oxidative stress in E. coli.

PubMed: 16216582
DOI: 10.1016/j.str.2005.07.007
主引用文献が同じPDBエントリー

実験手法

X-RAY DIFFRACTION (2.3 Å)