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1TYG

Structure of the thiazole synthase/ThiS complex

Summary for 1TYG
Entry DOI10.2210/pdb1tyg/pdb
DescriptoryjbS, Thiazole biosynthesis protein thiG, PHOSPHATE ION, ... (4 entities in total)
Functional Keywordsalpha beta barrel, protein-protein complex, this, thig, biosynthetic protein
Biological sourceBacillus subtilis
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Cellular locationCytoplasm (By similarity): O31618
Total number of polymer chains4
Total formula weight73846.38
Authors
Settembre, E.C.,Dorrestein, P.C.,Zhai, H.,Chatterjee, A.,McLafferty, F.W.,Begley, T.P.,Ealick, S.E. (deposition date: 2004-07-07, release date: 2004-09-28, Last modification date: 2024-02-14)
Primary citationSettembre, E.C.,Dorrestein, P.C.,Zhai, H.,Chatterjee, A.,McLafferty, F.W.,Begley, T.P.,Ealick, S.E.
Thiamin Biosynthesis in Bacillus subtilis: Structure of the Thiazole Synthase/Sulfur Carrier Protein Complex
Biochemistry, 43:11647-11657, 2004
Cited by
PubMed Abstract: Thiazole synthase is the key enzyme involved in the formation of the thiazole moiety of thiamin pyrophosphate. We have determined the structure of this enzyme in complex with ThiS, the sulfur carrier protein, at 3.15 A resolution. Thiazole synthase is a tetramer with 222 symmetry. The monomer is a (betaalpha)(8) barrel with similarities to the aldolase class 1 and flavin mononucleotide dependent oxidoreductase and phosphate binding superfamilies. The sulfur carrier protein (ThiS) is a compact protein with a fold similar to that of ubiquitin. The structure allowed us to model the substrate, deoxy-D-xylulose 5-phosphate (DXP), in the active site. This model identified Glu98 and Asp182 as new active site residues likely to be involved in the catalysis of thiazole formation. The function of these residues was probed by mutagenesis experiments, which confirmed that both residues are essential for thiazole formation and identified Asp182 as the base involved in the deprotonation at C3 of the thiazole synthase DXP imine. Comparison of the ThiS binding surface to the surface of ubiquitin identified a conserved hydrophobic patch of unknown function on ubiquitin that may be involved in complex formation between ubiquitin and one of its binding partners.
PubMed: 15362849
DOI: 10.1021/bi0488911
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (3.15 Å)
Structure validation

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