1TXD

Crystal Structure of the DH/PH domains of Leukemia-associated RhoGEF

1TXD の概要

• エントリーDOI: 10.2210/pdb1txd/pdb
• 分子名称: Rho guanine nucleotide exchange factor 12 (2 entities in total)
• 機能のキーワード: helical bundle (dh), beta sandwich (ph), signaling protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm (Probable): Q9NZN5
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 44694.29

構造登録者

Kristelly, R.,Gao, G.,Tesmer, J.J. (登録日: 2004-07-03, 公開日: 2004-09-21, 最終更新日: 2024-02-14)

主引用文献

Kristelly, R.,Gao, G.,Tesmer, J.J.
Structural determinants of RhoA binding and nucleotide exchange in leukemia-associated Rho guanine-nucleotide exchange factor.
J.Biol.Chem., 279:47352-47362, 2004

PubMed Abstract: Rho guanine-nucleotide exchange factors (RhoGEFs) activate Rho GTPases, and thereby regulate cytoskeletal structure, gene transcription, and cell migration. Leukemia-associated RhoGEF (LARG) belongs to a small subfamily of RhoGEFs that are RhoA-selective and directly activated by the Galpha12/13 family of heterotrimeric G proteins. Herein we describe the atomic structures of the catalytic Dbl homology (DH) and pleckstrin homology (PH) domains of LARG alone and in complex with RhoA. These structures demonstrate that the DH/PH domains of LARG can undergo a dramatic conformational change upon binding RhoA, wherein both the DH and PH domains directly engage RhoA. Through mutational analysis we show that full nucleotide exchange activity requires a novel N-terminal extension on the DH domain that is predicted to exist in a broader family of RhoGEFs that includes p115-RhoGEF, Lbc, Lfc, Net1, and Xpln, and identify regions within the LARG PH domain that contribute to its ability to facilitate nucleotide exchange in vitro. In crystals of the DH/PH-RhoA complex, the active site of RhoA adopts two distinct GDP-excluding conformations among the four unique complexes in the asymmetric unit. Similar changes were previously observed in structures of nucleotide-free Ras and Ef-Tu. A potential protein-docking site on the LARG PH domain is also evident and appears to be conserved throughout the Lbc subfamily of RhoGEFs.

PubMed: 15331592
DOI: 10.1074/jbc.M406056200

実験手法

X-RAY DIFFRACTION (2.13 Å)