1TW5

beta1,4-galactosyltransferase mutant M344H-Gal-T1 in complex with Chitobiose

Summary for 1TW5

Related1O0R 1TVY 1TW1
DescriptorBeta-1,4-galactosyltransferase 1, DI(N-ACETYL-D-GLUCOSAMINE), MANGANESE (II) ION, ... (8 entities in total)
Functional Keywordsmet344his mutation; closed conformation; chitobiose binding, transferase
Biological sourceBos taurus (cattle)
Cellular locationIsoform Long: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Isoform Short: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Processed beta-1,4-galactosyltransferase 1: Secreted P08037
Total number of polymer chains2
Total molecular weight68954.57
Authors
Ramakrishnan, B.,Boeggeman, E.,Qasba, P.K. (deposition date: 2004-06-30, release date: 2004-12-14, Last modification date: 2011-07-13)
Primary citation
Ramakrishnan, B.,Boeggeman, E.,Qasba, P.K.
Effect of the Met344His mutation on the conformational dynamics of bovine beta-1,4-galactosyltransferase: crystal structure of the Met344His mutant in complex with chitobiose
Biochemistry, 43:12513-12522, 2004
PubMed: 15449940 (PDB entries with the same primary citation)
DOI: 10.1021/bi049007+
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.3 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.251120.2%4.3%1.3%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 1tw5
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Molmil generated image of 1tw5
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Molmil generated image of 1tw5
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More Biological unit images

Molmil generated image of 1tw5
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Molmil generated image of 1tw5
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Molmil generated image of 1tw5
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(*)In the case of coarse surface representation, the asymmetric unit is shown as red ribbon representation.
Coordinate files for Biological unit (1tw5.pdb1.gz [54.65 KB])
Coordinate files for Biological unit (1tw5.pdb2.gz [54.48 KB])