1TW5

beta1,4-galactosyltransferase mutant M344H-Gal-T1 in complex with Chitobiose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Beta-1,4-galactosyltransferase 1	polymer	286	32856.5	2	UniProt (P08037) Pfam (PF13733) Pfam (PF02709)	Bos taurus (cattle)	Beta-1,4-GalTase 1; Beta4Gal-T1; b4Gal-T1
2	C, D, E (C, D, E)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
3	F, N (A, B)	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
4	G, O (A, B)	6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE	non-polymer		503.3	2	Chemie (UDH)
5	H, I, J, K, P (A, B)	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
6	L, Q (A, B)	1,4-DIETHYLENE DIOXIDE	non-polymer		88.1	2	Chemie (DIO)
7	M (A)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
8	R, S (A, B)	water	water		18.0	495	Chemie (HOH)

Sequence modifications

A, B: 130 - 402 (UniProt: P08037)

PDB	External Database	Details
Ala 117	-	SEE REMARK 999
Ser 118	-	SEE REMARK 999
Met 119	-	SEE REMARK 999
Thr 120	-	SEE REMARK 999
Gly 121	-	SEE REMARK 999
Gly 122	-	SEE REMARK 999
Gln 123	-	SEE REMARK 999
Gln 124	-	SEE REMARK 999
Met 125	-	SEE REMARK 999
Gly 126	-	SEE REMARK 999
Arg 127	-	SEE REMARK 999
Gly 128	-	SEE REMARK 999
Ser 129	-	SEE REMARK 999
Thr 342	Cys 269	engineered mutation
His 344	Met 271	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	65713.1
Branched	Number of molecules	3
	Total formula weight	1273.2
Non-Polymers*	Number of molecules	12
	Total formula weight	1968.3
All*	Total formula weight	68954.6

*Water molecules are not included.