1TV3

Crystal structure of the N-methyl-hydroxylamine MtmB complex

Summary for 1TV3

• Entry DOI: 10.2210/pdb1tv3/pdb
• Related: 1L2Q 1NTH
• Descriptor: Monomethylamine methyltransferase mtmB1, 5-(HYDROXY-METHYL-AMINO)-3-METHYL-PYRROLIDINE-2-CARBOXYLIC ACID (3 entities in total)
• Functional Keywords: tim barrel, transferase
• Biological source: Methanosarcina barkeri
• Total number of polymer chains: 1
• Total formula weight: 50357.31

Authors

Hao, B.,Zhao, G.,Kang, P.T.,Soares, J.A.,Ferguson, T.K.,Gallucci, J.,Krzycki, J.A.,Chan, M.K. (deposition date: 2004-06-26, release date: 2004-10-19, Last modification date: 2024-10-30)

Primary citation

Hao, B.,Zhao, G.,Kang, P.T.,Soares, J.A.,Ferguson, T.K.,Gallucci, J.,Krzycki, J.A.,Chan, M.K.
Reactivity and chemical synthesis of L-pyrrolysine- the 22(nd) genetically encoded amino acid
Chem.Biol., 11:1317-1324, 2004

PubMed Abstract: L-pyrrolysine, the 22(nd) genetically encoded amino acid, was previously deduced to be (4R, 5R)-4-substituted-pyrroline-5-carboxylate attached to the epsilon-nitrogen of lysine based on the crystal structure of the M. barkeri monomethylamine methyltransferase (MtmB). To confirm L-pyrrolysine's identity, structures of MtmB have been determined following treatment with hydroxylamine, N-methylhydroxylamine, or dithionite. Analysis of these structures has provided additional support for the presence of the pyrroline ring and, together with previous mass spectroscopy data, has led us to assign the C(4)-substituent to a methyl group. Based on this assignment, synthetic L-pyrrolysine was prepared by chemical methods. Detailed study of this chemically synthesized L-pyrrolysine has allowed us to characterize its physical properties, to study its chemical stability, and to elucidate the role of its C(4) substituent. Future applications of this synthetic L-pyrrolysine include its in vivo incorporation into recombinant proteins.

PubMed: 15380192
DOI: 10.1016/j.chembiol.2004.07.011

Experimental method

X-RAY DIFFRACTION (2.2 Å)